Table 1 Quantitative real-time PCR primers.
From: Oncogenic Role of the Ec Peptide of the IGF-1Ec Isoform in Prostate Cancer
Gene | Forward | Reverse |
|---|---|---|
PEc | 5′-TATCAGCCCCCATCTACCA-3′ | 5′-CTTGCGTTCTTCAAATGTACTTCCT-3′ |
IGF-1R | 5′-GGGAATGGAGTGCTGTATG-3′ | 5′-TCCTCCGCCTCCTGCTGCAGGTTCTT-3′ |
E-cadherin | 5′-TGGAGGAATTCTTGCTTTGC-3′ | 5′-CGTACATGTCAGCCAGCTTC-3′ |
Vimentin | 5′-GACAATGCGTCTCTGGCACGTCTT-3′ | 5′-TCCTCCGCCTCCTGCAGGTTCTT-3′ |
ZEB1 | 5′-TTCAGCATCACCAGGCAGTC-3′ | 5′-GAGTGGAGGAGGCTGAGTAG-3′ |