Figure 2

The PKR protein-binding function improves both cell viability and proliferation in pancreatic β cells. Twenty-four hours after transfection, MIN6 cells were treated with the indicated concentrations of coumermycin for the indicated times. (A) Cell viability was assessed by the MTT assay. (B) DNA synthesis was analyzed using EdU labeling assays. Representative micrographs of EdU labeling assays in MIN6 cells are shown (scale bar = 120 µm). Flow cytometric assays were performed with percentages of MIN6 cells at the (C) G1 phase and (D) S phase. (E) Isolated mouse islets were administered as MIN6 cells and DNA synthesis was analyzed using EdU labeling assays. In primary islets, insulin with green staining was used to mark β cells (scale bar = 100 µm), and the percentage of EdU-positive β cells was quantificated (using six islets for each group). Arrows indicate the EdU-positive β cell. Data are means ± SEM of three separate experiments. *P < 0.05 versus control.