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Figure 3 | Molecular Medicine

Figure 3

From: Protein-Binding Function of RNA-Dependent Protein Kinase Promotes Proliferation through TRAF2/RIP1/NF-κB/c-Myc Pathway in Pancreatic β cells

Figure 3

The PKR protein-binding function promotes cell cycle progression via upregulation of c-Myc transcription in MIN6 cells. Twenty-four hours after transfection, MIN6 cells were treated with coumermycin as indicated. (A) The mRNA levels of the indicated genes, including positive cell cycle regulators cyclin D1, D2, CDK2, CDK4 and c-Myc and negative regulators p18, p27 and p53 were determined by qRT-PCR. Cell extracts were analyzed by Western blotting with antibodies against (B) positive and (C) negative cell cycle regulators. si-c-Myc was cotransfected with GyrB-PKR-K296H into MIN6 cells, followed by treatment of coumermycin. (D) Quantification of EdU-positive β cell and percentages of MIN6 cells at the (E) G1 phase and (F) S phase were shown. Data are means ± SEM of three separate experiments. *P < 0.05 versus control.

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Molecular Medicine

ISSN: 1528-3658