Figure 4

NF-κB is required for PKR protein-binding-induced c-myc upregulation and cell proliferation in pancreatic β cells. After transfection, MIN6 cells were pretreated with Bay11-7082 (30 µmol/L, 2 h) and subsequently treated with coumermycin for 24 h. mRNA and protein levels of c-Myc were determined by (A) qRT-PCR and (B) Western blotting. (C) Quantification of EdU-positive β cell and percentages of MIN6 cells at the (D) G1 phase and (E) S phase were shown. (F) Isolated mouse islets were administered as panel C and DNA synthesis was analyzed using EdU labeling assays. The percentage of EdU-positive β cells in islets was quantificated (using six islets for each group). Arrows indicate the EdU-positive β cell. β-actin was detected as an internal control. Data are means ± SEM of three separate experiments. *P < 0.05 versus control.