Figure 2
From: Interleukin-9 Is Associated with Elevated Anti-Double-Stranded DNA Antibodies in Lupus-Prone Mice
IL-9 protein expression in spleens and kidneys of MRL/lpr mice. (A) IL-9 protein expression in B6 and MRL/lpr mouse spleens was assessed by immunohistochemical staining (100× original magnification). Increased magnification (400×) of the black-bordered box clearly shows the predominance of IL-9+ lymphocytes. (B) The numbers of IL-9+ lymphocytes in spleens of B6 and MRL/lpr mice were determined (n = 6 animals/group). The positive cells and surface area were counted under 400× original magnification and five randomly selected independent microscopic fields were counted for each sample to ensure that the data were representative and homogeneous. (C) IL-9 protein expression in kidneys of B6 and MRL/lpr mice was confirmed by immunohistochemical staining (100× original magnification). Increased magnification (400×) of the black-bordered box shows the predominance of IL-9+ lymphocytes. (D) The numbers of IL-9+ lymphocytes in kidneys of B6 and MRL/lpr mice were determined (n = 6 animals/group). The positive cells and surface area were counted under 400× original magnification and five randomly selected independent microscopic fields were counted for each sample to ensure that the data were representative and homogeneous.