Figure 3
From: Interleukin-9 Is Associated with Elevated Anti-Double-Stranded DNA Antibodies in Lupus-Prone Mice
IL-9 is associated with GC formation in spleens of MRL/lpr mice. (A) PNA protein expression in spleens from B6 and MRL/lpr mice was confirmed immunohistochemically (B) Numbers of PNA+ cells in spleens of B6 and MRL/lpr mice (n = 6 animals/group) were determined. Positive cells and surface area were counted under 400× magnification and five randomly selected independent microscopic fields were counted for each sample to ensure that the data were representative and homogeneous. (C) A positive correlation between IL-9+ cells and PNA+ cells in spleens of MRL/lpr mice was detected (n = 6 animals). (D) MRL/lpr mice were treated with vehicle or IL-9-neutralizing antibody every 4 wks for 12 wks. Numbers of PNA+ cells in spleens of MRL/lpr mice were determined (Right, n = 6 animals/group). Positive cells and surface area were counted under 400× magnification and five randomly selected independent microscopic fields were counted for each sample to ensure that the data were representative and homogeneous.