Figure 4
From: Interleukin-9 Is Associated with Elevated Anti-Double-Stranded DNA Antibodies in Lupus-Prone Mice
IL-9 induces B-cell proliferation and immunoglobulin production via activation of p-STAT3. (A) Naïve B cells isolated from MRL/lpr mice were cultured with LPS, anti-CD40 or anti-IgM in the presence of IL-9 or IL-9 + IL-17 for 2 d and the proliferation of B cells was analyzed with MTT. (B) Naïve B cells isolated from MRL/lpr mouse spleens were cultured with LPS, anti-CD40 or anti-IgM in the presence of IL-9 ± IL-17 for 3 d, and IgM and IgG secretion into supernatants was measured by ELISA. (C) Naïve B cells isolated from MRL/lpr mouse spleens were cultured with LPS, anti-CD40, anti-IgM or IL-9 + IL-17 in the presence or absence of 50 µmol/L SPI for 48 h, and cell proliferation was analyzed using the MTT assay. (D) Naïve B cells isolated from MRL/lpr mouse spleens were cultured with LPS, anti-CD40, anti-IgM or IL-9 + IL-17 in the presence or absence of SPI for 3 d, and IgM and IgG secretion into supernatants was measured by ELISA. These experiments were performed three times with similar results.