Figure 3

Alteration of insulin signaling cascades in skeletal muscle of rats after MI. Tyrosine phosphorylation of IR β-subunit and IRS, and serine phosphorylation of Akt (ser 473). Skeletal muscle tissue lysates were subjected to immunoprecipitation and/or Western blot using specific antibodies. Left panel, representative immunoblots are shown. Right panel, quantification of phosphorylation blots relative to their corresponding loading. Tyrosine phosphorylation of IR β-subunit relative to IR β-subunit, tyrosine phosphorylation of IRS1 relative to IRS1, serine phosphorylation of Akt (ser 473) relative to Akt. IP immunoprecipitation. IB, immunoblotting. I, insulin (with insulin stimulation). B, basal (without insulin stimulation). Values presented are means ± SEM of 4 to 5 samples in each group. *P < 0.05 versus control group with insulin stimulation. ^#P < 0.05 versus control group without insulin stimulation.