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Figure 1 | Molecular Medicine

Figure 1

From: Mouse Model for Protein Tyrosine Phosphatase D (PTPRD) Associations with Restless Leg Syndrome or Willis-Ekbom Disease and Addiction: Reduced Expression Alters Locomotion, Sleep Behaviors and Cocaine-Conditioned Place Preference

Figure 1

(A) Differential expression of PTPRD mRNA in cerebral cortex samples from individuals with different rs2381970 genotypes. Mean ± SEM of relative expression of PTPRD mRNA in cerebral cortical samples of individuals with major allele homozygote versus heterozygotes (n = 105 and 14, respectively). Triplicate RT-PCR assays. Relative expression was determined by the mean of two PTPRD amplimers in relation to the geometric mean of three control mRNAs from the same sample (see Materials and Methods). **p < 0.01. SNPs and nominal p values were as follows: rs10115782/0.00298; rs10815873/0.587; rs2296094/0.00905; rs35929428/0.418; rs956154/0.134; rs1975197/0.0513; rs17584499/0.883; rs7850165/0.646; rs973117/0.172; rs10511520/0.681; rs2381970/0.00162; rs4626664/0.0396; rs10977684/0.173; rs914804/0.513; rs3949639/0.701; rs17829640/0.273; rs1412875/0.235; rs10977801/0.773; rs12337005/0.0589; rs639168/0.927; rs17183242/0.599; rs7862769/0.289; rs12350467/0.391; rs10115039/0.316; rs7470838/0.0383; rs9886850/0.850; rs12345015/0.975; rs649891/0.316; rs1322307/0.967; rs1322313/0.973; rs1923442/0.609; rs10756064/0.741; rs10809140/0.109; rs10738180/0.906. (B) Cerebral cortical PTPRD mRNA levels in wild-type, heterozygous and homozygous knockout mice. Relative expression was determined by RT-PCR with primers complementary to sequences of exons 5′ to the site of deletion in the knockout (PTPRDa) and with a primer pair complementary to sequences within the deleted exon (PTPRDdel). Bars show mean ± SEM, n = 3. **p < 0.01, ***p < 0.001.

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Molecular Medicine

ISSN: 1528-3658