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Figure 7 | Molecular Medicine

Figure 7

From: Regulation of Vascular Tone, Angiogenesis and Cellular Bioenergetics by the 3-Mercaptopyruvate Sulfurtransferase/H2S Pathway: Functional Impairment by Hyperglycemia and Restoration by dl-α-Lipoic Acid

Figure 7

3-MP/3-MST mediated bioenergetic responses are suppressed in liver mitochondria of STZ-diabetic rats; restoration of the responses by DHLA in vitro. (A–D) Comparison of mitochondrial oxygen consumption rate (OCR) of liver mitochondria isolated from control (CTL) and streptozotocin-diabetic (STZ) rats, with or without the addition of 3-MP (10 µmol/L), in the presence or absence of preincubation with DHLA is shown in mitochondrial coupling assay experiments. (A,C) representative bioenergetic tracings; (B,D) collective OCR values (from six individual measurements conducted on different experimental days) show increments in the 3-MP-induced increases in bioenergetic responses (calculated as increments in area-under-the-curve (AUC) of the calculated respiratory reserve capacity responses after FCCP administration) in mitochondria isolated from control or STZ-diabetic rats, and in mitochondria of STZ-diabetic rats with or without DHLA (100 nmol/L) incubation. Note the suppression of the 3-MP induced bioenergetic responses in mitochondria from STZ-diabetic rats, and the stimulatory effect of DHLA in mitochondria isolated from STZ-diabetic (but not in mitochondria isolated from control) rats. Bar graphs show mean ± SEM values; **p < 0.01 shows significant differences between 3-MP treated and 3-MP combination with DHLA-treated diabetic mitochondria in vitro between the two indicated groups; NS: not significant.

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Molecular Medicine

ISSN: 1528-3658