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Figure 5 | Molecular Medicine

Figure 5

From: Angiotensin II Downregulates MicroRNA-145 to Regulate Kruppel-like Factor 4 and Myocardin Expression in Human Coronary Arterial Smooth Muscle Cells under High Glucose Conditions

Figure 5

Effect of miR-145 on Klf4 and myocardin protein expression under high glucose stimulation in cultured VSMCs. (A) Representative Western blots for Klf4 protein levels in VSMCs subjected to high glucose stimulation for 6 h in the absence or presence of WT miR-145, antagomir-145, mutant miR-145 and Klf4 and myocardin siRNA. (B) Quantitative analysis of Klf4 protein levels. The values from stimulated VSMCs have been normalized to values in control cells. #P < 0.01 versus control. n = 4 per group. (C) Representative Western blots for myocardin protein levels in VSMCs subjected to high glucose stimulation for 6 h in the absence or presence of WT miR-145, mutant miR-145, antogomir-145, scramble and Klf4 siRNA. (D) Quantitative analysis of myocardin protein levels. The values from stimulated VSMCs have been normalized to values in control cells. #P < 0.01 versus control. *P < 0.05 versus control. n = 4 per group.

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Molecular Medicine

ISSN: 1528-3658