Figure 5

NE phenotype, VEGF secretion and invasive capacity of LNCaP (A) and DU145 cells (B). Cells were treated for 6 d (LNCaP) or 4 d (DU145), with 50 µmol/L ISO, 10 nmol/L T3 or 100 nmol/L T4 and their respective combination with ISO. (A) Representative images by phase contrast microscope at 20× showing the presence of neurite-like projections (see arrows). Scale bar, 50 µm. (C) Representative immunoblot of pCREB and CREB and densitometric quantification in LNCaP cells. Brown adipose tissue (BAT) of cold-exposed rats was used as a positive control for pCREB. All data represent mean ± SEM and were analyzed by one-way ANOVA and post hoc Tukey tests. Different letters indicate differences with p < 0.05. n = 3−5 independent experiments.