Figure 3

IKK16 attenuates lung injury. Rats were subjected to HS and received IKK16 (1 mg/kg; i.v.) or vehicle (Veh) (10% DMSO) at resuscitation and 4 h later lungs were obtained. Sham animals were used as control. The representative images for the immunostaining for MPO as a neutrophils marker (A–D), CD68 as a macrophage marker (F–I), and the quantitative analysis of the number of cells/mm² (E and J) are shown. The scale bar represents 50 µm. The activities of MPO (K) and NAG (L) were also determined in lung tissue. The expression of ICAM-1 was determined by Western blotting. Densitometric analysis of the related bands is expressed as relative optical density, corrected for the corresponding β-actin contents, and normalized using the related mean value of sham-operated band (M). Data are presented as box and whiskers (minimum to maximum) and the central line represents the median (n = 5−6 animals per group). *p < 0.05 versus sham group and ^#p < 0.05 versus HS group.