Figure 5
IKK16 attenuates the phosphorylation of IKKαβ, IκBα and the nuclear translocation of the p65 NF-κB subunit. Rats were subjected to HS and received IKK16 (1 mg/kg; i.v) or vehicle (Veh) (10% DMSO) at resuscitation and 4 h later the phosphorylation of IKKαβ on Ser176/180 (A and D), IκBα on Ser32/36 (B and E) and the nuclear translocation of the p65 NF-κB subunit (C and F) on the kidney (A–C) and liver (D–F) were determined by Western blotting. Sham animals were used as control. Densitometric analysis of the related bands is expressed as relative optical density (O.D.), corrected for the corresponding β-actin contents, and normalized using the related mean value of sham-operated band. Data are presented as box and whiskers (minimum to maximum) and the central line represents the median (n = 3−4 animals per group). *p < 0.05 versus sham group and #p < 0.05 versus HS group.