Figure 8

IKK16 increases survival. Rats were subjected to HS and received IKK16 (1 mg/kg; i.v.) or vehicle (Veh) (10% DMSO) at resuscitation and 4 h later the phosphorylation of Ser⁴⁷³ and the total Akt on the kidney (A) and liver (B) were determined by Western blotting. Sham animals were used as control. Densitometric analysis of the related bands is expressed as relative optical density, corrected for the corresponding β-actin contents, and normalized using the related mean value of sham-operated band. Data are presented as box and whiskers (minimum to maximum) and the central line represents the median (n = 3−4 animals per group). *p < 0.05 versus sham group and ^#p < 0.05 versus HS group.

(C) HK-2 cells were serum deprived for 24 h and concomitantly incubated with indicates concentrations of IKK16 for the first 4 h. Cell viability was determined by methylthiazoletetrazolium assay and the absorbance was measured at 490 nm. Data are presented as box and whiskers (minimum to maximum) and the central line represents the median of the percentage of survival cells related to complete growth medium (DMEN) control group. *p < 0.05 versus DMEN group and ^#p < 0.05 versus serum-deprived group.