Figure 4From: Aloperine Protects Mice against Ischemia-Reperfusion (IR)-Induced Renal Injury by Regulating PI3K/AKT/mTOR Signaling and AP-1 ActivityTUNEL assay for analysis of tubular apoptosis. TUNEL assay and Western blot analysis of caspase 3 levels were employed for the assessment of tubular apoptosis. (A) Representative results for TUNEL assays of renal sections (magnification 400×). (B) Quantitative analysis of TUNEL positive cells as mean ± SEM of six mice analyzed for each group. (C) Representative results for Western blot analysis of caspase 3 levels in the kidneys. (D) Relative expression levels for caspase 3 by densitometric analysis as mean ± SEM of six mice analyzed for each group. **, P < 0.01; and P < 0.001.Back to article page