Figure 1

Candidate gene identification and placental expression throughout pregnancy. (A) Effect of EG-VEGF on homeobox gene expression profile in first-trimester placental explants (n = 6). Real-time PCR was used to detect homeobox gene mRNA relative to 18S rRNA. Results are reported as fold changes relative to untreated controls. (B) Quantification of NKX3.1 mRNA expression levels in placental tissues at 8 to 14 wks gestation (n = 12), 20 wks gestation (n = 3) and at term (n = 6). 18S rRNA was used as the internal control. Results are reported as the mean ± SEM. Data were analyzed using the Mann-Whitney U test (significant differences when compared with the 8 to 14 wks gestation period, *p < 0.05).