Figure 4From: An EG-VEGF-Dependent Decrease in Homeobox Gene NKX3.1 Contributes to Cytotrophoblast Dysfunction: A Possible Mechanism in Human Fetal Growth RestrictionEffect of in vitro differentiation on NKX3.1 mRNA expression in placental cells. (A) Quantification of NKX3.1 mRNA expression levels in primary VCT and EVCT cultures from first-trimester placentae (n = 12). 18S rRNA was used as an internal control. (B) Effects of forskolin (10 µmol/L, 72 h) on NKX3.1 mRNA expression levels in BeWo cells. 18S rRNA was used as an internal control. Results are reported as the mean ± SE mol/L from n = 3 independent experiments. Data were analyzed using the Mann-Whitney U test (significant differences, *p < 0.05).Back to article page