Figure 7

Candidate gene identification using human apoptosis cDNA array and placental expression for candidate gene in FGR and control pregnancies. Effect of NKX3.1 gene inactivation on apoptosis gene expression profile identified BCL2and BAXmRNA as candidate genes in BeWo and HTR-8/SVneo (A, B). Real-time PCR was used to detect apoptosis gene mRNA relative to 18S rRNA. Results are reported as fold changes relative to cells treated with NC. Quantification of candidate genes, BCL2and BAXmRNA expression levels in FGR (n = 25) and control (n = 25) (C, D). 18S rRNA was used as the internal control. Results are reported as the mean ± SEM. Data were analyzed using the Mann-Whitney U test (significant differences when compared with control, *p < 0.05; **p < 0.01).