Figure 4
From: Targeting Extracellular Cyclophilins Ameliorates Disease Progression in Experimental Biliary Atresia
MM284 treatment decreased SMAD2 phosphorylation. (A) The mice were euthanized on d 14 post-RRV infection, and portions of liver were harvested for protein extraction. Total protein lysates were used for PathScan Phospho-SMAD2 sandwich ELISA. Data represent absorbance at λ = 450 nm. Asterisks represent p values <0.05 between the RRV treated and control groups; the pound sign represents p values <0.05 between control and RRV + MM284 groups. (B) HSCs were treated with 800 ng/mL of Cyclophilin A (CypA) with or without 400 ng/mL of MM284. Cells were harvested at 72 h posttreatment and SMAD2/3 phosphorylation was analyzed in lysates by PathScan Phospho-SMAD2/3 sandwich ELISA. The experiment was performed in triplicate, and the error bars represent the standard deviations. Asterisks represent p values <0.01 between CypA treated and both control and CypA + MM284 groups.