Table 1 Primers.
From: Targeting Extracellular Cyclophilins Ameliorates Disease Progression in Experimental Biliary Atresia
Primers for proinflammatory cytokines | ||
|---|---|---|
Mediator | Forward primer (5′–3′) | Reverse primer (5′–3′) |
TNF-α | AGCCCCCAGTCTGTATCCTT | CTCCCTTTGCAGAACTCAGG |
IL-6 | AGTTGCCTTCTTGGGACTGA | TCCACGATTTCCCAGAGAAC |
IL-1 β | AGGCCACAGGTATTTTGTCG | GCCCATCCTCTGTGACTCAT |
Primers for fibrosis mediators | ||
Mediator | Forward primer (5′–3′) | Reverse primer (5′–3′) |
PAI-1 | CCGATCCTTTCTCTTTGTGG | TCAAGGCTCCATCACTTGG |
Integrin αVβ6 | GAACGCTCTAAGGCCAAGTG | TGCTTCTCCCTGTGCTTGTA |
TIMP-1 | GACCTATAGTGCTGGCTGTG | TCATCTTCCAGTTTGCAAGG |
TIMP-4 | TGCCAAATCACCACTTGCTA | ATAGAGCTTCCGTTCCAGCA |
MMP-7 | CCCTACCTATCAAAGAGACT | CAGCGTGTTCCTCTTTCCAT |
MMP-9 | CCTGGAACTCACACGACATC | GGAAACTCACACGCCAGAA |
GAPDH | GGCATTGCTCTCAATGACAA | CCCTGTTGCTGTAGCCGTAT |