Figure 2

Immunofluorescence staining of inflammatory markers in the liver, lungs and kidneys. Representative immunofluorescence staining images of (A) hepatic COX-2 expression and F4/80⁺ cell population in the sinusoids of the liver tissue, (B) HIF-1α expression and F4/80⁺ cell population in the renal glomeruli of the kidney tissue, and (C) PPAR-γ expression and Ly6G⁺ cell population in the lung alveoli (scale bars = 50 µmol/L). (D) Graphical representation of mean fluorescence intensities for proteins detected in each image were normalized against background intensities of the vehicle control group, expressed as arbitrary fluorescence units (A.U.) (N = 4, P < 0.05, ANOVA, post hoc LSD; values with different letters are significantly different).