Figure 4
From: Guizhi Fuling Wan as a Novel Agent for Intravesical Treatment for Bladder Cancer in a Mouse Model
GFW is harmless to normal bladder urothelium of C57BL/6 mice and reduces the hematuria caused by tumor growth in mouse bladders. (A) Female C57BL/6 mice aged 6 wks with no tumor implantation underwent treatment with PBS or GFW (180 mg/mL) 10 times, administered every other day. “AgNO3+GWF” indicates GFW-treated mice with initial AgNO3-induced chemical lesions on bladder urothelium. After the mice were sacrificed, the urinary system was isolated and photographed (upper panel). K, U and B indicate the kidney, ureter and bladder, respectively. Bladder sections were stained with H&E for histopathology. Magnification: 100× (middle panel) and 400× (bottom panel). In all cases, the bladders presented a normal appearance and uniform urothelium, indicating that even 10 consecutive treatments with GFW was insufficient to harm the urothelium of normal mouse bladders. (B) After implantation of MB49 murine bladder cancer cells, urine was inspected and photographed. Serious events of hematuria were observed in all of the untreated control mice on d 11 and 19, while only mild hematuria was observed in GFW-treated mice on d 11. This result indicates that GFW reduced the gross hematuria caused by tumor growth in mouse bladder. The arrow indicates hematuria.