Figure 5

Immunofluorescence studies using mouse tissues showing selective loss of STAT5a/b from SMA-positive tunica media (dark arrows: green staining) in main pulmonary artery (PA) (A) or PA segment in a lung section (B) of the conditional STAT5a/b^−/− mice. Quantitation is shown on the right of the respective panels; *P < 0.05. Scale bars = 10 µm. (C) Western blots of extracts of cultured arterial smooth muscle cells derived from pools of five mice, each of the indicated groups, with SMC-specific conditional homozygous deletion of STAT5a/b verifying the loss of expression of STAT5a (using L-20 pAb from Santa Cruz Biotechnology), STAT5a + b (using C-17 pAb from Santa Cruz Biotechnology) and male-biased expression of BCL6. Adapted from ref. (28). pAb, polyclonal antibody.