Figure 3
From: Alternative Mechanism for White Adipose Tissue Lipolysis after Thermal Injury
Burn and LPS increased lipolysis by inhibiting AMPK signaling in WAT. (A) Representative images of Western blot for phosphorylation of HSL at Ser565 and its upstream modulator of AMPK in WAT. (B–C) Quantitative densitometric analyses for Western blots in (A) Values are means ± SEM. N = 8 animals in each group. *P < 0.05 (one-way ANOVA, Bonferroni posttest). (D) Representative images of Western blots for perilipin, phospho-AMPK and phospho-HSL (Ser565) in in vitro differentiated 3T3-L1 adipocytes with or without pretreatment of 1 mmol/L metformin for 6 h and then challenged by 5 µg/mL tunicamycin and 100 ng/mL LPS for 3 or 6 h, respectively. (E–F) Quantitative densitometric analyses for Western blots in (D). Values are means ± SEM. *P < 0.05 (one-way ANOVA, Bonferroni posttest). In vitro experiments on 3T3-L1 adipocytes were repeated 3 times.