Figure 4
From: Alternative Mechanism for White Adipose Tissue Lipolysis after Thermal Injury
Burn plus LPS promoted apoptosis in WAT. (A) Representative images of immunofluorescent TUNEL staining of EWAT. Arrows indicate TUNEL-positive cells (magnification 200×). (B) Quantitative analysis of positive TUNEL-staining cells in EWAT. Values are means ± SEM. P < 0.05 in each paired comparison between groups except that between burn and LPS only (one-way ANOVA, Bonferroni posttest). (C) Correlate coefficient analysis between percentage of ORO-positive hepatocytes and percentage of TUNEL-positive cells in WAT. (D) Representative images of Western blots for proapoptotic signaling molecules including caspase-9, caspase-3, cleaved-caspase-3 and cleaved caspase-7. (E–H) Quantitative densitometric analyses for the above Western blots. Values are means ± SEM. *P < 0.05 (one-way ANOVA, Bonferroni posttest). N = 8 animals per group, N = 2 for histological observations in each group.