Figure 5
From: Alternative Mechanism for White Adipose Tissue Lipolysis after Thermal Injury
Burn plus LPS increased ER stress, which correlated with apoptosis in WAT. (A) RT-qPCR analysis of mRNA levels of Chop in EWAT. RT-qPCR data were normalized to 18s rRNA as an internal control. Values are means ± SEM. *P < 0.05 (one-way ANOVA, Bonferroni posttest). (B) Representative images of Western blots for ER stress markers (CHOP, ATF6) in WAT. (C–E) Quantitative densitometric analyses for the above Western blots. Values are means ± SEM. *P < 0.05 (one-way ANOVA, Bonferroni posttest). (F) Immunofluorescent double staining of cleaved caspase-3) and CHOP in WAT (magnification 400×). Arrows indicate CHOP positive cells. Arrowheads indicate cleaved caspase-3 positive cells. (G) Percentage of immunofluorescent positive cells in WAT in each treatment group. N = 8 animals per group, N = 2 for histological observations in each group.