Figure 6
From: Alternative Mechanism for White Adipose Tissue Lipolysis after Thermal Injury
Burn and LPS stimulated macrophage infiltration and inflammasome activation, which correlated with apoptosis in WAT. (A) Representative images of Western blots for NLRP3, caspase-1 and IL-1 β in WAT. (B-D) Quantitative densitometric analysis for Western blot of NLRP3, caspase-1 and IL-1 β in WAT. Values are means ± SEM. *P < 0.05 (one-way ANOVA, Bonferroni posttest). For IL-1 β, P value was calculated for 17 kDa mature form. (E) Immunofluorescent double staining of cleaved caspase-3 and MAC387 in WAT (magnification 400×). Arrows indicate MAC387 positive cells. Arrowheads indicate cleaved caspase-3 positive cells. (F) Percentage of immunofluorescent positive cells in WAT in each treatment group. N = 8 animals per group, N = 2 for histological observations in each group.