Figure 1
From: MicroRNA-15a/16 Regulates Apoptosis of Lung Epithelial Cells After Oxidative Stress
Effects of hyperoxia on miR-15a/16 levels. (A) Beas2B cells were exposed to room air (RA) or hyperoxia (95% O2, 5% CO2) for a time course between 6 and 48 h. miRNA was analyzed using real-time PCR. Figures represent three independent experiments with similar results. *p < 0.05. (B) C57BL/6J mice were exposed to RA or hyperoxia (100%). After 1–3 d, lung tissue miRNA was isolated and analyzed using real-time PCR (mean ± standard deviation [SD], n = 10). *p < 0.05. (C) C57BL/6J mice were exposed to RA or hyperoxia (100%). After 1–3 d, BALF miRNA was analyzed using real-time PCR (mean ± SD, n = 10). PCR (mean ± SD, n = 10). *p < 0.05. (D) Beas2B cells were incubated with RA, H2O2 (100 µmol/L), hyperoxia (95% O2, 5% CO2) or H2O2 (100 µmol/L) + hyperoxia (95% O2, 5% CO2). After 6 h, miRNA was analyzed using real-time PCR. Figures represent three independent experiments with similar results. *p < 0.05. (E) Beas2B cells were incubated with RA, H2O2 (40 µmol/L) or hyperoxia (95% O2, 5% CO2). After a designated time point, miRNA was analyzed using real-time PCR (mean ± SD, n = 3). *p < 0.05. (F) Beas2B cells were exposed to RA or hyperoxia (95% O2, 5% CO2) in the absence or presence of NAC (5 mmol/L). After 6 h, miRNA was analyzed using real-time PCR. Figures represent three independent experiments with similar results. *p < 0.05.