Figure 2

Suppression of miR-15a/16-induced apoptosis after hyperoxia. Beas2B cells were grown to approximately 70% confluence and transfected with negative controls or miR-15a/16 inhibitors. After 12 h, the cells were exposed to RA or hyperoxia (95% oxygen). After another 24 h, the cells were harvested for flow cytometry analysis (A) or terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining (B). (A) Apoptosis of Beas2B cells was analyzed using flow cytometry. Statistical analysis is shown on the right. Figures represent three independent experiments with similar results. *p < 0.05. (B) TUNEL staining of Beas2B cells after hyperoxia. Apoptotic nuclei were labeled by TUNEL (black arrow). Statistical analysis is shown on the right. Figures represent three independent experiments with similar results. *p < 0.05. (C) AECs were isolated from hybrid mice (mice after crossbreeding C57BL/6J WT mice with CC10 Cre mice serve as control; mice after crossbreeding miR15a/16^−/− loxP mice with CC10 Cre mice serve as lung epithelial cell-specific miR-15a/16^−/− mice). AEC were cultured for 6 d and then were incubated in room air (RA) (21% oxygen) or hyperoxia (95% oxygen) for 24 h. Next, the cells were harvested and detected using FACS. Statistical analysis is shown on the right. Figures represent two independent experiments with the similar results. *p < 0.05.