Figure 5
From: MicroRNA-15a/16 Regulates Apoptosis of Lung Epithelial Cells After Oxidative Stress
Suppression of miR-15a/16 modulated both intrinsic and extrinsic pathways, detected using IHC in vivo. Lung epithelial cell-specific miR-15a/16−/− mice and control mice (described in Figure 2 legend) were exposed to hyperoxia (right panels in the figures) or room air (RA; left panels in the figures). After 3 d, lung tissue sections were obtained. Bcl-2, caspase-3, caspase-8 and Bad were detected using IHC staining. Positive cells were stained with DAB (×200). Statistical analysis is shown on the right. Figures represent two independent experiments with similar results. *p < 0.05.