Figure 4

CLL mouse model. (A) CLL PBMCs from two patients were labeled with CFSE and randomized among the groups. The 10⁸ CFSE-labeled cells were injected intravenously into NSG mice (lacking T, B and NK cells). Groups of five mice received daily dosing of saline: NXT629 at 30 mg/kg IP or fludarabine at 50 mg/kg IP. Mice were killed 4 wks after engraftments, and the splenocytes were stained with hCD19 and analyzed by flow cytometry. Data are hCD19⁺/CFSE⁺ cells normalized to vehicle control. (B) Proliferative model. Groups of mice received daily dosing of saline, NXT629 at 30 mg/kg IP. Mice were killed 2 wks after engraftments of CLL cells. CLL cells in spleen of NSG mice were quantified by gating on human CD45⁺ cells, and a second gate was applied to determine CD19⁺/CD5⁺ CLL cells. Representative flow cytometry plots are depicted to the left. The absolute number of CLL cells is significantly decreased in NXT629-treated animals. Data are mean ± SEM. Significant difference: *p < 0.05, unpaired Student t test. n.s., Nonsignificant.