Figure 5
Daily administration of galantamine decreases islet infiltration by immune cells. (A) Insulitis scoring scheme examplars. Islets with no infiltrating mononuclear cells were scored as 0, peri-islet inflammation only = 1, moderate intra-islet inflammation occupying <70% of the islet = 2, severe-complete intra-islet inflammation occupying >70% of the islet = 3. (B, C) Pancreata from 16-wk-old NOD mice injected intraperitoneally with saline or galantamine from 5 wks of age were isolated and frozen in OCT media and then sliced at 10 µm. At least 50 total islets from three disparate areas of each pancreas were scored blindly. Administration of galantamine (B) reduced the severity of insulitis in NOD mice (representative images), as well as (C) improved the overall level of insulitis in the pancreas (n = 5, χ2 p < 0.0005).