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Figure 2 | Molecular Medicine

Figure 2

From: Simvastatin Inhibits Toll-like Receptor 8 (TLR8) Signaling in Primary Human Monocytes and Spontaneous Tumor Necrosis Factor Production from Rheumatoid Synovial Membrane Cultures

Figure 2

Simvastatin inhibits TLR8 signaling in HEK Blue TLR8 cells, which is not reversed by treatment with GGPP. (A) HEK Blue TLR8 cells (which stably express TLR8 and a reporter gene expressing a SEAP under the control of a NF-κB/AP-1-inducible promoter) were cultured for 30 min in the presence of 10 µg/mL simvastatin ± 10 µmol/L GGPP, before stimulation with 5 µg/mL R-848 for 16 h. Supernatants were analyzed for SEAP activity by using the Quanti-Blue assay and cell viability (B) was measured by MTT assay. Data are representative of three independent experiments.

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Molecular Medicine

ISSN: 1528-3658