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Figure 4 | Molecular Medicine

Figure 4

From: Deletion of β-Arrestin2 in Mice Limited Pancreatic β-Cell Expansion under Metabolic Stress through Activation of the JNK Pathway

Figure 4

The effect of βarr2 on β-cell proliferation. (A) Representative micrographs of BrdU and Ki67 staining in islets of βarr2+/+ and βarr2−/− mice. Scale bar: 50 µm. Arrows: BrdU- or Ki67-positive cells. (B) Quantification of BrdU- or Ki67-positive cells in the total islet cells (n = 3−5). (C) Cell cycle distribution analyzed by flow cytometry in INS-1 (832/13) cells infected with adenovirus expressing GFP or βarr2 and exposed to 3 mmol/L (G3) or 11 mmol/L (G11) glucose. (D) Relative mRNA transcriptional levels of cell cycle genes from INS-1 (832/13) cells with βarr2 overexpression (n = 6−8). Expression of mRNA was normalized with β-actin. (E) Cyclin D2 and p21 protein levels in INS-1 (832/13) cells infected with adenovirus expressing GFP or βarr2. Pictures are representative of four independent experiments. *P < 0.05, **P < 0.01, versus control.

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Molecular Medicine

ISSN: 1528-3658