Figure 5

βarr2 protected β-cell against high glucose- and free fatty acids-induced apoptosis. (A, B) Islets isolated from 16-wk-old βarr2^+/+ and βarr2^−/− mice and INS-1 (832/13) cells infected with adenoviruses expressing GFP or βarr2 were exposed to 6.1 mmol/L (G6.1) or 30 mmol/L (G30) glucose, or BSA alone or with 0.4 mmol/L palmitate (PA0.4). Representative fluorescence micrographs after double staining with propidium iodide (red) and Hoechst 33324 (blue) of islets (A) and INS-1 (832/13) cells (B) from five independent experiments are shown. Scale bar: 200 µm. (C) Quantitative percentage of apoptotic cells in the total INS-1 (832/13) cells (n = 5). **P < 0.01 versus GFP.