Figure 3

Low oxygen tension increases the protein expression of EPOR but not of the β common receptor. (A) Immunostaining of BAECs cultured under 21% oxygen or 5% oxygen (24 h incubation) with anti-EPOR and anti-βCR antibodies, which were then examined by confocal microscopy. Nuclei were stained with DAPI. Images shown are at 20× magnification with insets representing a 100× magnification of the cells. Negative control images represent cells that have not been treated with the antibodies against the protein of interest. Quantitative analysis of EPOR and βCR expression is shown in (B) represented as corrected total cell fluorescence (arbitrary unit). Each data point represents the mean ± SEM (n = 3), **p < 0.01.