Figure 2
From: Treatment with Anti-HMGB1 Monoclonal Antibody Does Not Affect Lupus Nephritis in MRL/lpr Mice
Anti-HMGBl mAb treatment of MRL/lpr mice does not affect plasma levels of HMGB1, anti-dsDNA antibodies and complement C3, while increasing HMGB1 antibody levels. (A) Plasma anti-dsDNA (units/mL) was measured by ELISA in MRL/MPJ (n = 10) and MRL/lpr (n = 10–12) mice at wks 7, 9, 13 and 17. (B) Plasma complement C3 (mg/mL) was measured by ELISA in MRL/MPJ (n = 10) and MRL/lpr (n = 10–12) mice at wk 7, 9, 13 and 17. (C) Plasma HMGB1 was measured by Western blotting in MRL/MPJ (n = 10) and MRL/lpr (n = 10–12) mice at wks 7, 9, 11, 13, 15 and 17. HMGB1 levels are presented as values of fluorescence intensity and were normalized against a standard of Jurkat cell lysate. (D) Plasma anti-HMGB1 (ng/mL) was measured by ELISA in MRL/MPJ (n = 10) and MRL/lpr (n = 10–12) mice at wks 7, 9, 11, 13, 15 and 17. Anti-HMGB1 levels are presented as values of fluorescence intensity and were normalized against a standard of monoclonal anti-HMGB1 antibody. Depicted are the median values with range at each time point.