Figure 1
From: Human γδ T Cells Augment Antigen Presentation in Listeria Monocytogenes Infection
Human γδ T cells possess phagocytic capacity. (A) FCM analysis of γδ T cells and αβ T cells before incubation with L. monocytogenes. (A1) The percentage of γδ T cells in PBMCs at 10 d culture with RPMI 1640 medium containing IL-2 after stimulation in anti-pan-TCRγδ mAb-coated plates for five days. (A2) The percentage of αβ T cells after stimulation with CD3 and CD28 antibodies for four days. (A3) FCM analysis of naïve γδT cells sorted from fresh isolated PBMCs. (B) Representative TEM images of L. monocytogenes phagocytosis. (B1) Activated γδ T cells displayed “hairy” appearance after 10 d culture with anti-γδ TCR antibody and IL-2. (B2) Activated γδ T cells packaged L. monocytogenes bacterium by pseudopod-like plasma membrane after one hour of co-culture. (B3) Activated γδ T cells phagocytized L. monocytogenes three hours after incubation. (B4) Necrotic γδ T cells with L. monocytogenes in plasma membrane after co-culture for five hours. (B5) Rested γδ T cells display small but similar phagocytosis. (B6) Naïve γδ T cells did not phagocytize L. monocytogenes. (B7) Activated αβ T cells did not uptake L. monocytogenes. Scale bars = 0.5 µm.