Figure 3

FCM analysis of antigen presentation related markers on γδ T cells in response to L. monocytogenes infection. (A) The level of HLA-DR expression increased on rested γδ T cells six hours after incubation with L. monocytogenes. (B) Quantification of normalized mean fluorescence intensities (MFI) of HLA-DR expression on rested γδ T cells in different groups. R represents the ratio of bacteria number to γδ T cell number. The high ratio of bacterium-to-cell of 50:1 (blue line) induced more HLA-DR expression on rested γδ T cells compared with low ratio (red line) or PBS (black line). No significant changes were observed at 12 h and15 h time points. (C, D, E, F, G and H) No significant change was observed in the expression level of HLA-DR (C and D), CD80 (E and F) or CD86 (G and H) on activated γδ T cells either in the presence or absence of L. monocytogenes. Data are shown as mean ± SEM (Independent experiments: n = 5).