CCL4, CCL5, CXCL9, CXCL10, CCR5 and CXCR3 mRNA expression in spleens of mice immunized twice with NP with or without TAK-779 treatment. Individual spleen pieces from different groups of mice were collected 2 wks after TAK-779 treatment. The mRNA expression of CCL4 (A), CCL5 (B), CXCL9 (C), CXCL10 (D), CCR5 (E) and CXCR3 (F) was measured by quantitative real-time RT-PCR. Results are normalized to β-actin expression. Results are expressed as the mean ± SD. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001. The data are representative of 2 independent experiments with 4 mice per group. Mice were analyzed individually.