Effector functional characteristics of cells in NP-immunized mice with or without TAK-779 treatment compared with control group. Two weeks after TAK-779 cessation, individual spleens were collected from naïve mice, mice treated with TAK-779 (TAK), mice immunized once (NP) or twice with NP (NP2) and mice immunized once (TN) or twice (TN2) with NP and treated with TAK-779. Splenocytes were prepared and analyzed by IFN-γ ELISPOT assay upon restimulation with NP protein. Results are expressed as the mean ± SD of the number of positive spots per 2 × 105 cells. Results are representative of 2 independent experiments. * p ≤ 0.05, ** p ≤ 0.001.