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Figure 1 | Molecular Medicine

Figure 1

From: Reduction of Cellular Expression Levels Is a Common Feature of Functionally Affected Pendrin (SLC26A4) Protein Variants

Figure 1

Functionality of wild-type pendrin and seven variants identified in Brazilian deaf patients. (A, B) Average intracellular fluorescence intensity measured in HEK 293 Phoenix cells transfected with wild-type (WT) or mutated pendrin and EYFP p.H148Q;I152L or EYFP p.H148Q;I152L alone (control) and bathed in chloride and iodide containing solutions. Arrows indicate the addition of iodide to the extracellular solution. Fluorescence intensity was normalized for the average of fluorescence intensity in high chloride solution. (C, D) Percentage of fluorescence decrease (ΔF%) determined over the experimental period (19 s) for the pendrin variants indicated in (A) and (B). ***p < 0.001, n.s., not statistically significant, one-way ANOVA with Bonferroni multiple comparison posttest.

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Molecular Medicine

ISSN: 1528-3658