Figure 5

Abundance of wild-type pendrin and pendrin variants in the plasma membrane region of living cells. (A) Wild-type (WT) and mutated pendrin-EYFP (green signal) were expressed in HeLa cells together with ECFP (blue signal) as an indicator of the transfection efficiency of the single cell. Living cells were stained with a suitable dye to clearly identify the plasma membrane (magenta signal). Scale bar: 25 µm. (B) Wild-type (WT) and mutated pendrin fluorescence intensity in three regions of interest of the plasma membrane of a single cell were expressed as levels of gray, averaged and normalized for the fluorescence intensity of ECFP in the cytosol of the same cell. n = 16, **p < 0.01, *p < 0.05 compared with wild-type, one-way ANOVA with Dunnett multiple comparison posttest.