Figure 4From: Generation of HLA-Universal iPSC-Derived Megakaryocytes and Platelets for Survival Under Refractoriness ConditionsMegakaryocytes (MKs) differentiated from HLA-universal iPSCs remain silenced for HLA class I. (A) Relative levels of β2m transcripts in shNS- or shβ2m- expressing and nontransduced MKs assessed by real time PCR. (B) Surface expression of HLA class I molecules in nontransduced, nonsilenced and silenced MKs analyzed by flow cytometry. Cells were harvested and stained with HLA-specific antibody w6/32. Both graphs depict means and SD of three independent experiments. ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05.Back to article page