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Figure 5 | Molecular Medicine

Figure 5

From: A Stromal Cell-Derived Factor 1α Analogue Improves Endothelial Cell Function in Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome

Figure 5

SDF-1α and CTCE inhibited thrombin-induced permeability through miR-126. HMVECs were transfected with miR-126 inhibitors or control inhibitors and treated with thrombin (0.01 U), SDF-1α (250 ng/mL), CTCE (10 µg/mL) or a combination of thrombin with SDF-1α (A) or CTCE (B). HMVEC permeability was determined by measuring electrical impedance using an ECIS system. Thrombin decreased HMVEC monolayer integrity (*p < 0.05) and this effect was significantly reduced by treatment with SDF-1α (A) and CTCE (B) (#p < 0.05). Transfection with a miR-126 inhibitor alone did not affect thrombin-induced permeability; however, concomitant treatment with SDF-1α (A) or CTCE (B) demonstrated that the miR-126 inhibitor partially but significantly blocked their protective effects ($p < 0.05). Repeated-measures ANOVA used for all comparisons. N = 5 independent experiments.

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Molecular Medicine

ISSN: 1528-3658