Figure 2
From: Increased Risk of Interstitial Lung Disease in Children with a Single R288K Variant of ABCA3
Induction of cellular multidrug resistance in vitro by ABCA3. (A) Effects of soluble doxorubicin on viability of HEK293 cells stably expressing ABCA3-WT or ABCA3-R288K protein. Different concentrations of doxorubicin were used and viability was measured with XTT assay. Values are displayed as means ± SEM of HEK293 cells stably expressing either ABCA3 wild-type protein (solid line) or R288K mutated ABCA3 protein (dashed line). *P < 0.05; ****P < 0.0001. (B) Light microscopy of HEK293 cells stably expressing ABCA3-WT and ABCA3-R288K protein respectively. Additionally cells were treated with 10 µmol/L doxorubicin for 3 h and displayed after 24 h of growth in RPMI medium supplemented with 10% FCS under normal conditions.