Figure 4
From: Increased Risk of Interstitial Lung Disease in Children with a Single R288K Variant of ABCA3
Determination of vesicle volumes in A549 cells with ABCA3-WT or ABCA3-R288K protein. (A) Immunofluorescence staining of HA-tagged ABCA3 vesicles imaged with a confocal microscope. Shown is either ABCA3-WT (left) or ABCA3-R288K containing vesicles (right). (B) Volumes of ABCA3 containing vesicles harboring either ABCA3-WT or ABCA3-R288K were calculated on the basis of vesicle diameters determined with ImageJ software tool. ***P < 0.001.