Figure 2

Plumbagin inhibits PKM2 expression in activated macrophages. (A–C) Indicated macrophages were treated with lipopolysaccharide (LPS) (100 ng/mL) in the absence or presence of plumbagin for 24 h. The mRNA or protein levels of PKM2 (A–B) or HK-1 (C) were assayed using Q-PCR and western blot, respectively (n = 3, *, p < 0.05 versus LPS group). (D) Native gel electrophoresis was performed using whole-cell extracts from BMDMs after treatment with LPS (100 ng/mL) and/or plumbagin (3 µmol/L) for 24 h.