Figure 3

NOX4 is required for PKM2 expression in glycolysis. (A) Western blot analysis of NOX4 expression in control and PKM2 shRNA-treated bone marrow-derived macrophages (BMDMs) and peritoneal macrophages (PMs). (B) Indicated macrophages were treated with lipopolysaccharide (LPS) (100 ng/mL) for 24 h. Relative reactive oxygen species (ROS), PKM2 mRNA, and lactate were assayed (n = 3, *, p< 0.05 versus control shRNA group). (C) Indicated macrophages were treated with LPS (100 ng/mL) in the absence or presence of GKT137831 (1 µmol/L) for 24 h. PKM2 mRNA and lactate levels were assayed (n = 3, *, p < 0.05 versus LPS group).